Research Methods and Protocols

Protocols and Methods by Scientific Paradigm

Contents 1 Biology and Biochemistry 1.1 Structural & Physiological Biology 1.2 Protein Assays 1.3 DNA & RNA Assays 2 Cognitive and Behavioral 3 Computational and Informatics 4 Imaging 4.1 Brain Imaging 5 Comments

Biology and Biochemistry

Structural & Physiological Biology

• Histology
• Stereology
• DIG non-radioactive in situ hybridisation
• Radioactive in situ hybridisation

Protein Assays

• Quantitative Autoradiography
• Membrane Fractions for Western Blot

DNA & RNA Assays

Real-time Reverse Transcriptase Polymerase Chain Reaction (PCR)

Cognitive and Behavioral

Learning & Memory

• Morris Water Maze - Working Memory Test
• Spatial Delayed Response (SDR)Test
• Spontaneous Alternation Test

Conditioning

• Trace Eye Blink

Motor

• Parallel Bar Motor Coordination Test

Computational and Informatics

Needed

• Matlab Techniques

Imaging

Brain Imaging

Needed

• Structural (MRI)
• Functional (fMRI)
• Diffusion Tensor Imaging (DTI)
• Functional Connectivity
• Arterial Spin Labeling (ASL)
• Using AFNI
• Using FSL

Comments